engineered protein Search Results


elisa kit for vegf  (R&D Systems)
93
R&D Systems elisa kit for vegf
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
Elisa Kit For Vegf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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engineered amaranth protein g  (Biomol GmbH)
90
Biomol GmbH engineered amaranth protein g
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
Engineered Amaranth Protein G, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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site-directed engineering of defined chromosomal sites for recombinant protein and virus expression  (Biopharm GmbH)
90
Biopharm GmbH site-directed engineering of defined chromosomal sites for recombinant protein and virus expression
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
Site Directed Engineering Of Defined Chromosomal Sites For Recombinant Protein And Virus Expression, supplied by Biopharm GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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site-directed engineering of defined chromosomal sites for recombinant protein and virus expression - by Bioz Stars, 2026-06
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protein probe search engine  (Micromass UK Limited)
90
Micromass UK Limited protein probe search engine
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
Protein Probe Search Engine, supplied by Micromass UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein engineering market  (Zion Market Research)
90
Zion Market Research protein engineering market
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
Protein Engineering Market, supplied by Zion Market Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein engineering  (Genentech inc)
90
Genentech inc protein engineering
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
Protein Engineering, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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– center for protein engineering  (InBios International)
90
InBios International – center for protein engineering
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
– Center For Protein Engineering, supplied by InBios International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody development and protein engineering  (MedImmune llc)
90
MedImmune llc antibody development and protein engineering
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
Antibody Development And Protein Engineering, supplied by MedImmune llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein engineered variants of naturally occurring wild type proteases kannase  (Novozymes limited)
90
Novozymes limited protein engineered variants of naturally occurring wild type proteases kannase
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
Protein Engineered Variants Of Naturally Occurring Wild Type Proteases Kannase, supplied by Novozymes limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodydiscovery & protein engineering  (AstraZeneca ltd)
90
AstraZeneca ltd antibodydiscovery & protein engineering
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
Antibodydiscovery & Protein Engineering, supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dept. of antibody engineering  (Genentech inc)
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Genentech inc dept. of antibody engineering
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
Dept. Of Antibody Engineering, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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engineered protein modules  (IMDEA Food)
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IMDEA Food engineered protein modules
Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the <t>VEGF</t> mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.
Engineered Protein Modules, supplied by IMDEA Food, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the VEGF mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.

Journal: Acta biochimica et biophysica Sinica

Article Title: MiR-20b targets AKT3 and modulates vascular endothelial growth factor-mediated changes in diabetic retinopathy.

doi: 10.1093/abbs/gmw065

Figure Lengend Snippet: Figure 1. Effects of high glucose-induced miR-20b down-regulation in HRECs (A) Effects of HG on miR-20b expression. MiR-20b expression was suppressed by HG exposure; transfection of miR-20b restored the expression levels of miR-20b in HG-treated HRECs; transfection of miR-20b inhibitor also reduced the expression level of miR-20b in control HRECs. MiR-20b levels were analyzed by qRT-PCR. (B) Effects of HG on the VEGF mRNA expression. VEGF mRNA expression was up-regulated in HRECs when exposed to HG; transfection of miR-20b suppressed the levels of VEGF mRNA in HG-treated HRECs; transfec- tion of miR-20b inhibitor increased the expression levels of VEGF mRNA in control HRECs, VEGF mRNA levels were analyzed by qRT-PCR. (C) Permeability assay in HRECs with different treatments. (D) Angiogenesis assay in HRECs with different treatments. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.

Article Snippet: VEGF protein analysis VEGF protein levels were determined by using a commercially available ELISA kit for VEGF (R&D Systems, Minneapolis, USA) according to the manufacturer’s instruction.

Techniques: Expressing, Transfection, Control, Quantitative RT-PCR, Permeability, Angiogenesis Assay

Figure 3. AKT3 mediates the regulation of VEGF and functional changes in HG-treated HRECs (A,B) Transfection of AKT3 siRNA in HG-treated HRECs sup- pressed the expression level of AKT3 mRNA and protein. (C,D) Transfection of AKT3 siRNA in HG-treated HRECs reduced the expression level of VEGF mRNA and protein. (E,F) Transfection of AKT3 siRNA reduced permeability and angiogenesis in HG-treated HRECs. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.

Journal: Acta biochimica et biophysica Sinica

Article Title: MiR-20b targets AKT3 and modulates vascular endothelial growth factor-mediated changes in diabetic retinopathy.

doi: 10.1093/abbs/gmw065

Figure Lengend Snippet: Figure 3. AKT3 mediates the regulation of VEGF and functional changes in HG-treated HRECs (A,B) Transfection of AKT3 siRNA in HG-treated HRECs sup- pressed the expression level of AKT3 mRNA and protein. (C,D) Transfection of AKT3 siRNA in HG-treated HRECs reduced the expression level of VEGF mRNA and protein. (E,F) Transfection of AKT3 siRNA reduced permeability and angiogenesis in HG-treated HRECs. Data are presented as the mean ± SEM, n = 3. *P < 0.05, **P < 0.01.

Article Snippet: VEGF protein analysis VEGF protein levels were determined by using a commercially available ELISA kit for VEGF (R&D Systems, Minneapolis, USA) according to the manufacturer’s instruction.

Techniques: Functional Assay, Transfection, Expressing, Permeability

Figure 4. Differential expressions of miR-20b, VEGF mRNA and AKT3 mRNA in the retina and RECs of non-diabetic and diabetic rats Relative expres- sions of miR-20b in non-diabetic control and diabetic rat retina tissue sam- ples (A) and RECs (B) were analyzed by qRT-PCR. Relative expressions of VEGF mRNA in non-diabetic control and diabetic rat retina tissue samples (C) and RECs (D) were analyzed by qRT-PCR. Relative expressions of AKT3 mRNA in non-diabetic control and diabetic rat retina tissue samples (E) and RECs (F) were analyzed by qRT-PCR. Data are presented as the mean ± SEM, n = 6. **P < 0.01, ***P < 0.001.

Journal: Acta biochimica et biophysica Sinica

Article Title: MiR-20b targets AKT3 and modulates vascular endothelial growth factor-mediated changes in diabetic retinopathy.

doi: 10.1093/abbs/gmw065

Figure Lengend Snippet: Figure 4. Differential expressions of miR-20b, VEGF mRNA and AKT3 mRNA in the retina and RECs of non-diabetic and diabetic rats Relative expres- sions of miR-20b in non-diabetic control and diabetic rat retina tissue sam- ples (A) and RECs (B) were analyzed by qRT-PCR. Relative expressions of VEGF mRNA in non-diabetic control and diabetic rat retina tissue samples (C) and RECs (D) were analyzed by qRT-PCR. Relative expressions of AKT3 mRNA in non-diabetic control and diabetic rat retina tissue samples (E) and RECs (F) were analyzed by qRT-PCR. Data are presented as the mean ± SEM, n = 6. **P < 0.01, ***P < 0.001.

Article Snippet: VEGF protein analysis VEGF protein levels were determined by using a commercially available ELISA kit for VEGF (R&D Systems, Minneapolis, USA) according to the manufacturer’s instruction.

Techniques: Control, Quantitative RT-PCR

Figure 5. AKT3 mediates the regulation of VEGF in the diabetic rat retina In the diabetic rat received miR-20b-NC or miR-20 mimic treatment, miR-20b (A), VEGF mRNA (B), and AKT3 mRNA (D) expression levels were determined by qRT-PCR. (C) VEGF protein expression level was determined by ELISA assay. (E) AKT3 and other isoforms of AKT protein expression level was determined by western blot analysis. In the diabetic rat received AKT3-siRNA-NC or AKT3- siRNA treatment, AKT3 mRNA (F) and protein (G) expression levels were determined by qRT-PCR and western blot analysis, respectively; and VEGF mRNA (H) and protein (I) expression levels were determined by qRT-PCR and ELISA assay, respectively. Data are presented as the mean ± SEM, n = 6. *P < 0.05, **P < 0.01.

Journal: Acta biochimica et biophysica Sinica

Article Title: MiR-20b targets AKT3 and modulates vascular endothelial growth factor-mediated changes in diabetic retinopathy.

doi: 10.1093/abbs/gmw065

Figure Lengend Snippet: Figure 5. AKT3 mediates the regulation of VEGF in the diabetic rat retina In the diabetic rat received miR-20b-NC or miR-20 mimic treatment, miR-20b (A), VEGF mRNA (B), and AKT3 mRNA (D) expression levels were determined by qRT-PCR. (C) VEGF protein expression level was determined by ELISA assay. (E) AKT3 and other isoforms of AKT protein expression level was determined by western blot analysis. In the diabetic rat received AKT3-siRNA-NC or AKT3- siRNA treatment, AKT3 mRNA (F) and protein (G) expression levels were determined by qRT-PCR and western blot analysis, respectively; and VEGF mRNA (H) and protein (I) expression levels were determined by qRT-PCR and ELISA assay, respectively. Data are presented as the mean ± SEM, n = 6. *P < 0.05, **P < 0.01.

Article Snippet: VEGF protein analysis VEGF protein levels were determined by using a commercially available ELISA kit for VEGF (R&D Systems, Minneapolis, USA) according to the manufacturer’s instruction.

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot